三、主要科研工作与成绩 主持科研项目： (1) 科技部国家重点研发计划项目：有机碳一原料高效利用和转化人工合成细胞的构建(课题2：有机碳一原料高效生物利用机制研究)。 (2) 国家自然科学基金：双功能蛋白BirA在食气永达尔梭菌化能固碳中的作用机制研究。 (3) 上海市2021年度“科技创新行动计划”科技支撑碳达峰碳中和专项：工业含碳尾气生物转化制长碳链化合物的关键技术研发、集成与示范。 (4) 中国科学院洁净能源创新研究院（国家重点实验室）合作基金：合成气生物发酵制乙醇的关键技术研究、集成与示范。。 (5) 国家自然科学基金：小蛋白介导的梭菌溶剂合成调控机制研究。 (6) 上海市2017年度“科技创新行动计划”专项：二氧化碳高效转化利用的合成生物学研究。 (7) 中国科学院青年创新促进会优秀会员基金：一碳气体生物转化关键技术研究。
主要论文： 1. Tian, J.*, Deng W., Zhang Z., Xu J., YangG., Zhao G., Yang S., Jiang W.*, Gu Y.* (2023)Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery. Nature Communications, 14, 7758. 2. Tian, J., Li, C., Dong, Z., Yang, Y., Xing, J., Yu, P., Xin, Y., Xu, F., Wang, L., Mu, Y. Guo, X. Sun, Q., Zhao, G.,Gu, Y. *, Qin, G* and Jiang, W. * (2023) Inactivation of the antidiabetic drug acarbose by human intestinal microbial-mediated degradation. Nature Metabolism, 5, 896-909. 3.Liu, Y., Zhang, Z., Jiang, W.* and Gu, Y.* (2022) Protein acetylation-mediated cross regulation of acetic acid and ethanol synthesis in the gas-fermenting Clostridium ljungdahlii.The Journal of Biological Chemistry, 298, 101538. 4.Liu, Y., Zhang, Z., Zuo, N., Jiang, W.* and Gu, Y.* (2023) Programmable acetylation modification of bacterial proteins by a Cas12a-Guided acetyltransferase. ACS Synthetic Biology, 12, 2135-2146. 5.Zhao, R., Dong, W., Yang, C., Jiang, W., Tian, J.* and Gu, Y.* (2023) Formate as a supplementary substrate facilitates sugar metabolism and solvent production by Clostridium beijerinckii NCIMB 8052. Synthetic and Systems Biotechnology, 8, 196-205. 6.Zhang, C., Nie, X., Zhang, H., Wu, Y., He, H., Yang, C., Jiang, W.* and Gu, Y.* (2021) Functional dissection and modulation of the BirA protein for improved autotrophic growth of gas-fermenting Clostridium ljungdahlii. Microbial Biotechnology, 14, 2072-2089. 7.Yang, G., Hong, S., Yang, P., Sun, Y., Wang, Y., Zhang, P., Jiang, W.* and Gu, Y.* (2021) Discovery of an ene-reductase for initiating flavone and flavonol catabolism in gut bacteria. Nature Communications, 12, 790. 8.Jia, D., He, M., Tian, Y., Shen, S., Zhu, X., Wang, Y., Zhuang, Y., Jiang, W. and Gu, Y.* (2021) Metabolic engineering of gas-fermenting Clostridium ljungdahlii for Efficient Co-production of Isopropanol, 3-Hydroxybutyrate, and Ethanol. ACS Synthetic Biology, 10, 2628-2638. 9.Zhang, L., Zhao, R., Jia, D., Jiang, W.* and Gu, Y.*(2020) Engineering Clostridium ljungdahlii as the gas-fermenting cell factory for the production of biofuels and biochemicals. Current Opinion in Chemical Biology, 59, 54-61. 10.Zhang, L., Liu, Y., Zhao, R., Zhang, C., Jiang, W.* and Gu, Y.*(2020) Interactive regulation of formate dehydrogenase during CO2 fixation in gas-fermenting bacteria. mBio, 11 (4): e00650-20. 11.Yang, Y., Zhang, H., Lang, N., Zhang, L., Chai, C., He, H., Jiang, W.* and Gu, Y.* (2020) The small RNA sr8384 is a crucial regulator of cell growth in solventogenic clostridia. Applied and Environmental Microbiology, 86 (13): e00665-20. 12.Yang, Y., Lang, N., Zhang, L., Wu, H., Jiang, W.* and Gu, Y.* (2020) A novel regulatory pathway consisting of a two-component system and an ABC-type transporter contributes to butanol tolerance in Clostridium acetobutylicum. Applied Microbiology and Biotechnology, 104, 5011-5023. 13.Liu, Z.Y., Jia, D.C., Zhang, K.D., Zhu, H.F., Zhang, Q., Jiang, W.H., Gu, Y.* and Li, F.L.* (2020) Ethanol metabolism dynamics in Clostridium ljungdahlii grown on carbon monoxide. Applied and Environmental Microbiology, 86 (14): e00730-20. 14.Zhao, R., Liu, Y., Zhang, H., Chai, C., Wang, J.*, Jiang, W.* and Gu, Y.* (2019) CRISPR-Cas12a-mediated gene deletion and regulation in Clostridium ljungdahlii and its application in carbon flux redirection in synthesis gas fermentation. ACS Synthetic Biology, 8, 2270-2279. 15.Huang, H., Chai, C., Yang, S., Jiang, W.* and Gu, Y.* (2019) Phage serine integrase-mediated genome engineering for efficient expression of chemical biosynthetic pathway in gas-fermenting Clostridium ljungdahlii. Metabolic Engineering, 52, 293-302. 16.Zhang, L., Liu, Y., Yang, Y., Jiang, W.* and Gu, Y.* (2018) A novel dual-cre motif enables two-way autoregulation of CcpA in Clostridium acetobutylicum. Applied and Environmental Microbiology, 84 (8): e00114-18. 17.Yang, Y., Nie, X., Jiang, Y., Yang, C.*, Gu, Y.*and Jiang, W.* (2018) Metabolic regulation in solventogenic clostridia: regulators, mechanisms and engineering. Biotechnology Advances, 36, 905-914. 18.Yang, Y., Zhang, L., Huang, H., Yang, C., Yang, S., Gu, Y.* and Jiang, W.* (2017) A flexible binding site architecture provides new insights into CcpA global regulation in gram-positive bacteria. mBio, 8 (1):e02004-16. 19.Yang, G., Jia, D., Jin, L., Jiang, Y., Wang, Y., Jiang, W. and Gu, Y*. (2017) Rapid generation of universal synthetic promoters for controlled gene expression in both gas-fermenting and saccharolytic Clostridium species. ACS Synthetic Biology, 6, 1672-1678. 20.Zhang, Y., Xu, S., Chai, C., Yang, S., Jiang, W., Minton, N.P. and Gu, Y. (2016) Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum. FEMS Microbiology Letters, 363, fnw065. 21.Yang, Y., Lang, N., Yang, G., Yang, S., Jiang, W.* and Gu, Y.* (2016) Improving the performance of solventogenic clostridia by reinforcing the biotin synthetic pathway. Metabolic Engineering, 35, 121-128. 22.Xue, Q., Yang, Y., Chen, J., Chen, L., Yang, S., Jiang, W.* and Gu, Y.* (2016) Roles of three AbrBs in regulating two-phase Clostridium acetobutylicum fermentation. Applied Microbiology and Biotechnology, 100, 9081-9089. 23.Ren, C., Wen, Z., Xu, Y., Jiang, W.* and Gu, Y.* (2016) Clostridia: a flexible microbial platform for the production of alcohols. Current Opinion in Chemical Biology, 35, 65-72. 24.Huang, H., Chai, C., Li, N., Rowe, P., Minton, N.P., Yang, S., Jiang, W. and Gu, Y*. (2016) CRISPR/Cas9-based efficient genome editing in Clostridium ljungdahlii, an autotrophic gas-fermenting bacterium. ACS Synthetic Biology, 5, 1355-1361. 25.Wu, Y., Yang, Y., Ren, C., Yang, C., Yang, S., Gu, Y.*and Jiang, W.* (2015) Molecular modulation of pleiotropic regulator CcpA for glucose and xylose coutilization by solvent-producing Clostridium acetobutylicum. Metabolic Engineering, 28, 169-179. 26.Sun, Z., Chen, Y., Yang, C., Yang, S., Gu, Y.* and Jiang, W.* (2015) A novel three-component system-based regulatory model for d-xylose sensing and transport in Clostridium beijerinckii. Molecular Microbiology, 95, 576-589.
主要专利： 1. 合成3-羟基丁酸的食气梭菌，中国，专利号：202311277850.1 2. 提高梭菌合成气利用的方法，中国，专利号：202311076870.2 3. 提升梭菌气体发酵性能的方法，中国，专利号：202211608492.3 4. 构建高效利用甲酸的大肠杆菌工程菌的方法，中国，专利号：202210564130.2 5. 构建高效利用甲酸的重组需钠弧菌的方法，中国，专利号：202211209238.6 6. 利用甲酸合成靛蓝素的需钠弧菌，中国，专利号：202211209216.X 7. 一种细菌体内靶向蛋白酰基化修饰的方法，中国，专利号：202111383529.2 8. 丙酮丁醇梭菌中的一个非编码RNA及其应用，中国，专利号：201810794247.3 9. 一种提高合成气产乙醇含量的突变株和利用突变株的应用，中国，专利号：ZL202110435996.2 |
